Urea Agar and Urease Test Broth are used for the differentiation of organisms, especially the Enterobacteriaceae, on the basis of urease production.
Urease Test Broth was developed by Rustigian and Stuart. It may be used for the identification of bacteria on the basis of urea utilization and it is particularly recommended for the differentiation of members of the genus Proteus from those of Salmonella and Shigella in the diagnosis of enteric infections. The medium is positive for Proteus, Morganella morganii subsp. morganii, Providencia rettgeri, and a few Providencia stuartii strains with the reclassification of the members of the Proteeae.