Sabouraud Dextrose Broth is used for culturing yeasts, molds and aciduric microorganisms. The medium is rendered more selective for fungi by the addition of antimicrobics.
SUMMARY AND EXPLANATION
Sabouraud Dextrose Broth is used for culturing yeasts and molds in cosmetics. General Chapter <62> of the USP recommends the use of Sabouraud Dextrose Broth when isolating Candida albicans from nonsterile pharmaceutical products.
Sabouraud dextrose media are peptone media supplemented with dextrose to support the growth of fungi. Sabouraud agar is also available with maltose substituted for the dextrose. Peptones are sources of nitrogenous growth factors. The carbohydrate provides an energy source for the growth of microorganisms. Gentamicin is an aminoglycoside antibiotic that inhibits the growth of gram-negative bacteria.