Description

INTENDED USE

LB Agar, Miller and LB Broth, Miller (Luria-Bertani) are used for maintaining and propagating Escherichia coli in molecular microbiology procedures.

SUMMARY AND EXPLANATION

LB Agar, Miller and LB Broth, Miller are based on LB Medium as described by Miller for the growth and maintenance of E. coli strains used in molecular microbiology procedures. These are nutritionally rich media designed for growth of pure cultures of recombinant strains. E. coli grows more rapidly because they provide the cells with amino acids, nucleotide precursors, vitamins and other metabolites that the microorganism would otherwise have to synthesize.

LB Broth, Miller contains twenty times the sodium chloride level of Luria Broth Base, Miller and twice the level found in LB Broth, Lennox. This allows the researcher to select the optimal salt concentration for a specific strain in LB Agar, Miller.

PRINCIPLE

Peptone provides nitrogen and carbon. Vitamins (including B vitamins) and certain trace elements are provided by yeast extract. Sodium ions for transport and osmotic balance are provided by sodium chloride. Agar is the solidifying agent in LB Agar, Miller.