Description
INTENDED USE
Tryptic Soy Broth w/ Lecithin and Polysorbate 80 is a broth medium recommended for use in quantitative procedures for the detection and enumeration of microorganisms present on surfaces of sanitary importance.
SUMMARY AND EXPLANATION
In 1948, Weber and Black reported lecithin, added to a broth medium in sufficient concentration, would effectively neutralize quaternary ammonium compounds.1 In further testing, Brummer combined lecithin and polysorbate 80 in an agar medium and reported it adequately neutralized disinfectants.2 Tryptic Soy Broth (TSB) is prepared according to the formula for Soybean-Casein Digest Broth recommended in the United States Pharmacopeia (USP).3 TSB w/ Lecithin and Polysorbate 80 is also used in environmental air sampling procedures.
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