Description
INTENDED USE
Enterobacteriaceae Enrichment Mossel broth is intended for the selective enrichment of Enterobacteriaceae. The undesired accompanying bacterial flora is inhibited by brilliant green and ox bile. Glucose favors the growth of all Enterobacteriaceae, and the high buffering capacity of the culture medium prevents the formed acid from killing the culture (2). Glucose is the main energy source and supports the rapid growth of all Enterobacteriaceae, while the enzymatic digestion of animal tissue provides nitrogen, vitamins, and amino acids (3).
SUMMARY AND EXPLANATION
EE Broth Mossel Enrichment is prepared according to the formula of Mossel, Visser, and Cornelissen (4). The formula contains dextrose to facilitate the growth of most Enterobacteriaceae, thus ensuring the detection of Salmonella and other lactose-negative organisms. EE Broth Mossel Enrichment should be used as an enrichment broth, followed by a selective medium; e.g., Violet Red Bile Agar. The enumeration of Enterobacteriaceae is of great concern in monitoring the sanitary condition of food. Enterobacteriaceae can be injured in food-processing procedures, which include exposure to low temperatures, sub-marginal heat, drying, radiation, preservatives, or sanitizers (5). Recovery relies on proper results-citation of damaged cells. EE Broth Mossel Enrichment is used to detect and enumerate Enterobacteriaceae found per milliliter or per gram of test sample of food when performing the Most Probable Number (MPN) technique with pre-enrichment (6, 7) EE Broth Mossel Enrichment is listed in the USP as one of the recommended media for the isolation of bile-tolerant gram-negative bacteria from nonsterile pharmaceutical products.
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