CBSAlife Water Examination kit simultaneously detects total coliforms and E. coli O157 in water. It is based on CBSAlife ColiNutrient’s proprietary. When total coliforms metabolize CBSAlife ColiNutrient indicator, ONPG, the sample turns yellow. When E. coli O157 metabolize CBSAlife ColiNutrient indicator, MUG, the sample also fluoresces. CBSAlife Water Examination kit can simultaneously detect these bacteria at 1 cfu/100 mL within 24 hours even with as many as 2 million heterotrophic bacteria per 100 mL present.