Brain Heart Infusion (BHI) is a general-purpose liquid medium used in the cultivation of fastidious and nonfastidious microorganisms, including aerobic and anaerobic bacteria, from a variety of clinical and nonclinical materials. It serves as a base for supplemented media containing 0.1% agar, Fildes enrichment or 6.5% sodium chloride. A supplemented pre-reduced formulation in tubes is especially recommended for the cultivation of anaerobes.
SUMMARY AND EXPLANATION
Rosenow described brain-heart infusion broth prepared by adding pieces of brain tissue to meat infusion or beef extractdextrose broth. A variation of this medium appeared for many years in the National Formulary. The current formulation is similar to the NF Brain Heart Infusion Broth, but the brain infusion component is composed of solids resulting from the drying of the liquid material and the heart infusion component has been replaced with a peptone of partially digested animal tissue.
BHI broth, 0.5 mL per tube, is used for the cultivation of bacteria employed in the preparation of inocula for microdilution minimal inhibitory concentration (MIC) and identification (ID) test panels. When a large number of cells are inoculated into the small volume of broth, a bacterial culture rapidly reaches its stationary phase of growth. The medium is also used in 5-mL amounts per tube for the preparation of inocula in antimicrobial susceptibility test procedures. This volume and the 8-mL tubes also can be used for general purposes.
BHI Broth is a nutritious, buffered culture medium that contains infusions of brain and heart tissue and peptones to supply protein and other nutrients necessary to support the growth of fastidious and nonfastidious microorganisms.