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Brain Heart Infusion (BHI) is a general-purpose liquid medium used in the cultivation of fastidious and nonfastidious microorganisms, including aerobic and anaerobic bacteria, from a variety of clinical and nonclinical materials. It serves as a base for supplemented media containing 0.1% agar, Fildes enrichment or 6.5% sodium chloride. A supplemented pre-reduced formulation in tubes is especially recommended for the cultivation of anaerobes.

BHI broth is used for the cultivation of a wide variety of microorganisms, including bacteria, yeasts and molds. BHI broth, 0.5 mL per tube, is used for the cultivation of bacteria employed in the preparation of inocula for microdilution minimal inhibitory concentration (MIC) and identification (ID) test panels.