Tryptic (Trypticase) Soy Broth (Soybean-Casein Digest Medium) is a general purpose medium used in qualitative procedures for the cultivation of fastidious and nonfastidious microorganisms from a variety of clinical and nonclinical specimens.
SUMMARY AND EXPLANATION
Tryptic (Trypticase) Soy Broth (TSB) is a nutritious medium that will support the growth of a wide variety of microorganisms, including common aerobic, facultative and anaerobic bacteria and fungi. This formulation is included in the USP as a medium for use in performing microbial enumeration tests and tests for specified microorganisms when testing nonsterile pharmaceutical products.
TSB was chosen by the USDA Animal and Plant Health Inspection Service for detecting viable bacteria in live vaccines. TSB is recommended for testing bacterial contaminants in cosmetics and complies with established standards in the food industry.
Because of its capacity for growth promotion, TSB is also recommended for use as the inoculum broth for disc diffusion and agar dilution antimicrobial susceptibility testing as standardized by the Clinical and Laboratory Standards Institute (CLSI).
Enzymatic digests of casein and soybean provide amino acids and other complex nitrogenous substances. Dextrose is an energy source. Sodium chloride maintains the osmotic equilibrium. Dibasic potassium phosphate acts as a buffer to control pH.