Description

CBSAlife Buffered Peptone Water is used for preenriching injured Salmonella species from food specimens to increase recovery.

Edel and Kampelmacher1 noted that food preservation techniques involving heat, desiccation, preservatives, high osmotic pressure, or pH changes cause sublethal injury to salmonellae. Preenrichment in a nonselective medium allows for the repair of cell damage and facilitates the recovery of salmonellae. Lactose Broth is frequently used for this purpose but it may be detrimental to recovering salmonellae. Buffered Peptone Water maintains a high pH over the pre-enrichment period and results in the repair of injured cells that may be sensitive to low pH. This is particularly important for vegetable specimens that have a low buffering capacity. Buffered Peptone Water can be used for testing dry poultry feed. Test methods have been published for a variety of food samples.

These pre-enrichment media contain peptone as a source of carbon, nitrogen, vitamins, and minerals. Sodium chloride maintains the osmotic balance. Phosphates buffer the media.