Blood culture biphasic containing solid agar and a liquid phasewas developed to circumvent the necessity of making repeat subcultures. After inoculation, the flask is supplemented with 10% CO2 and tilted so that the liquid covers the solid medium and incubated in the upright position. Flasks are examined every 3 days for the presence of colonies. If no growth is observed, flasks are tilted again and re-incubated, and the cycle is repeated for at least 35 days.
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