RPMI1640 With L-Glutamine and 25mM HEPES Buffer
RPMI1640 one of the proposed medium with a wide range of applications for mammalian cells, especially for hematopoietic cells. RPMI is a modified version of McCoys 5A and used to cultivate peripheral blood lymphocytes. RPMI1640 is prepared of Sodium bicarbonate buffer for the cultivation of many mammalian cells in typical conditions (PH=8). RPMI1640 supports the growth of a wide variety of cells in soluble and single-layer state. If this medium comes with a serum or a suitable replacement for the serum and It can be used as a functional culture medium for mammalian cells, including human lymphocytes, integrated protocols and hybrid cell growth.