Williams’ Medium E
In 1971, Williams et al. described a procedure for enriching isolated hepatocyte cultures for polygonal epithelial cells and reducing the number of contaminating fibroblasts. This method was a sequential plating technique based on the observation that fibroblast-like cells adhere to a substrate more rapidly than epithelial cells. The isolated epithelial cells resulting from this treatment could then be cultured in a rich medium designated Williams′ Medium D. Newborn animals were the source of cells used in these studies, as they were in most studies of liver cell culture. Since newborn liver is not functionally mature, further studies were conducted by Williams and Gunn to explore the possibility of culturing adult liver on a long-term basis. The medium developed during the course of these studies was designated Williams′ Medium E. It has been shown to support the growth in long-term culture of adult liver epithelial cells.
William’s E Medium contains no proteins or growth factors. Therefore, William’s E Medium requires supplementation, commonly with 5-10% Fetal Bovine Serum (FBS). William’s E Medium uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
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