Waymouth Medium MB 752/1 was developed as a totally defined synthetic medium for cultivation of mouse L929 cells in a serum-free environment. Clones of L929 cells, conditioned to grow in serum supplemented or serum-free medium were cultivated for more than thirty passages in serum-free Waymouth Medium and demonstrated a doubling time of approximately 24 hours. The applicability of Waymouth Medium MB 752/1 has been extended to include whole organ culture, establishment of carcinoma cell lines from pleural effusions, and the growth of potentially tumorigenic cells prior to their assessment in vivo.
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