Media 199

M-199 Medium was originally designed for nutritional studies to promote continuous growth of primary chick embryo heart and fibroblast cells without serum supplementation. Today, 199 media are widely used for the maintenance of non-transformed cells, vaccine and virus production and primary explants of epithelial cells. Long term cellular culture requires addition of a serum. When properly supplemented, Medium 199 provide broad applicability across a wide range of cell species, specifically for non-transformed cells.

The media can be formulated either with Earle salts or Hanks Salts. The media formulated with the Earle salts are buffered with a bicarbonate / carbonic acid solution and retain their pH in a CO2 incubator. The use of the Earle salts under ambient conditions results in a rapid rise in the pH of the culture medium.

The media formulated with the Hanks salts are buffered with saline solutions designed for balancing in ambient conditions and their use in a CO2 incubator results in a rapid drop in the pH of the culture medium.

Additional information

Weight 0.5 g
Theoretical pH

7.3 ± 0.3


288 mOsm/kg ± 10%


Red, orange clear solution

Storage conditions

+2°C to +8°C, protected from light

Shelf life

18 months

Sterility tests

– Bacteria in aerobic and anaerobic conditions
– Fungi and yeasts


< 1 EU/ml


Displayed on website; also available on request


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