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MCDB media was designed for the low-protein or serum-free growth of specific cell types using hormones, growth factors, trace elements or low levels of dialyzed fetal bovine serum protein. Each MCDB medium was formulated to provide a defined and optimally balanced nutritional environment that selectively promoted growth of a specific cell type.

Biological Industries’ MCDB Modified 153 supports general cell culture and the growth of normal human keratinocytes if supplemented with EGF, Insulin, Hydrocortisone, Ethanolamine, and Phosphoethanolamine.

MCDB 105 and 110 are modifications of MCDB 104 medium. These modifications are optimized for long-term survival and rapid clonal growth of human diploid fibroblast-like cells (WI-38, MRC-5, IMR-90) and of low-passage human foreskin fibroblasts using FBSP or hormone and growth factor supplements.

MCDB 151 is a modification of the medium Ham’s F-12, designed for the growth of human keratinocytes cells using low levels of dialyzed FBS or no serum protein.

The selection of a nutrient medium is strongly influenced by 1] type of cell, 2] type of culture [monolayer, suspension, clonal] and 3] degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.