CAD 22.00

The F-12 Medium, also known as Ham’s F-12, was formulated by R. G. Ham (1965) for the synthetic, serum-free culturing of single-cell Chinese hamster ovary (CHO) cells. F-12 is based on Ham’s F-10 medium with increased concentrations of choline, inositol, putrescine and amino acids. A key change over the predecessor medium F-10 is increased zinc, which greatly improved plating efficiency and reproducibility for CHO cells. F-12 was designed primarily for the plating of single cells and not for supporting populations exceeding 100,000 cells. However, it is now widely used for a variety of mammalian cell types. Depending on the type of culture, F-12 may require serum or protein supplementation. Nutrient Mixture F-12 is also the medium of choice for a Clonal Toxicity Assay (CTA) and has been utilized with low density, serum-free growth of Chinese Hamster Ovary (CHO) cells. F-12 is frequently used with dialysed serum, hormones, selenium and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbit and rat and has proved to be an excellent cloning medium for the culture of myeloma and hybrid cells.

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Additional information

Theoretical pH

7.3 ± 0.3


297 mOsm/kg ± 10 %


salmon – pink, clear solution

Storage conditions

+2°C to +8°C

Shelf life

12 months

Sterility tests

– Bacteria in aerobic and anaerobic conditions
– Fungi and yeasts


< 1 EU/ml

Indications of deterioration

Medium should be clear and free of particulate and flocculent material. Do not use if medium
is cloudy or contains precipitate.
Other evidence of deterioration may include color change or degradation of physical or
performance characteristics.


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