CAD 22.00

Ham’s F-10 was first developed by R. G. Ham (1962) for supporting single-cell growth of a diploid Chinese hamster ovary (CHO) cell line. This medium was found to be superior to an existing medium, F7, for both serum-free growth of CHO and for low-serum requirements of other mammalian cell lines. The key features include a higher amount of arginine, low tryptophan, proline and a variety of other nutrients such as thiamine, hypoxanthine and zinc. Depending on the application, commercial formulations of Ham’s F-10 may come with or without serum supplementation. Ham’s F-10 is known not only to support primary explants of chicken, rabbit and rat tissues, but also the growth of White Blood Cells (WBC’s) for chromosomal analysis and the growth of human diploid cells. F10 Nutrient Mixture (Ham’s) provides greater reliability and improved consistency in mammalian cell culture resulting in better overall efficiency.

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Additional information

Theoretical pH

7.3 ± 0.3


285 mOsm/kg ± 10 %


pink – orange, clear solution

Storage conditions

+2°C to +8°C

Shelf life

12 months


< 1 EU/ml

Sterility tests

– Bacteria in aerobic and anaerobic conditions
– Fungi and yeasts

Indications of deterioration

Medium should be clear and free of particulate and flocculent material. Do not use if medium
is cloudy or contains precipitate.
Other evidence of deterioration may include color change or degradation of physical or
performance characteristics.


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