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Glasgow Minimum Essential Medium was originally developed by lan MacPherson and Michael Stoker as a modification of Eagle′s medium and by modifying Eagle′s BME by adding 10% tryptose phosphate (e.g., Sigma Prod. No. T8159 at 100 ml/L medium) and twice the normal concentration of amino acids and vitamins. This medium was used to study the genetic factors affecting cell competence. The polyoma virus was used to transform four fibroblast clones from a culture of baby hamster kidney cells.
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| Theoretical pH | 7.5 ± 0.5 |
|---|---|
| Osmolality | 310 mOsm/kg ± 10 % |
| Color | red solution |
| Storage conditions | +2°C to +8°C |
| Shelf life | 12 months |
| Sterility tests | – bacteria aerobic-anaerobic |
| Endotoxin | < 1 EU/ml |
| Recommended use | – Respect storage conditions of the product |
| Indications of deterioration | Medium should be clear and free of particulate and flocculent material. Do not use if medium is cloudy or contains precipitate. |
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