GMEM


22.00 $

Glasgow Minimum Essential Medium was originally developed by lan MacPherson and Michael Stoker as a modification of Eagle′s medium and by modifying Eagle′s BME by adding 10% tryptose phosphate (e.g., Sigma Prod. No. T8159 at 100 ml/L medium) and twice the normal concentration of amino acids and vitamins. This medium was used to study the genetic factors affecting cell competence. The polyoma virus was used to transform four fibroblast clones from a culture of baby hamster kidney cells.

10 in stock

Additional information

Theoretical pH

7.5 ± 0.5

Osmolality

310 mOsm/kg ± 10 %

Color

red solution

Storage conditions

+2°C to +8°C

Shelf life

12 months

Sterility tests

– bacteria aerobic-anaerobic
– bacteria strictly anaerobic
– fungi / yeast

Endotoxin

< 1 EU/ml

Indications of deterioration

Medium should be clear and free of particulate and flocculent material. Do not use if medium is cloudy or contains precipitate.
Other evidence of deterioration may include colour change or degradation of physical or performance characteristics.

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