Differential Coliform Agar is a selective medium used for the detection of Escherichia coli in samples using the membrane filtration method in a laboratory setting. Tryptose and Proteose Peptone provide nitrogen, carbon, and amino acids in DC Medium. Yeast Extract supplies vitamins and minerals. Sodium Chloride maintains the osmotic balance of the medium. Bile Salts is a selective agent against Gram-positive bacteria, particularly bacilli and fecal streptococci. Cefsulodin supplements the medium as a selective agent. Neutral Red is the dye indicator, and Agar is the solidifying agent.